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Optogenetics – how to start using it?

Optogenetics – how to start using it?

Time: TBA

Place: TBA

Number of Participants: 12

Trainers: Kacper Kondrakiewicz, MA (Laboratory of Neurobiology of Emotions, Nencki Institute of Experimental Biology)

 

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Note: Only participants who registered for the Conference are eligible to attend the workshops.

 

During the last 15 years, optogenetics has pushed forward the limits of how precisely can we manipulate the nervous system. Although this technique is relatively straightforward and could be applied in many laboratories, some people still perceive it as complicated and very expensive. During my workshop, I will try to prove that this is not true. I will briefly explain the theoretical foundations of this technique and describe what is needed to start using it. The participants will learn how the light can be delivered into tissue, what equipment is necessary for this technique and how its costs can be reduced to a few thousands (or even hundreds) of euros. Everyone will have occasion to prepare an optical cannula and measure its power.

Kacper Kondrakiewicz (Laboratory of Neurobiology of Emotions, Nencki Institute of Experimental Biology, Polish Academy of Science) is a PhD student in the Nencki Institute of Experimental Biology, Warsaw, Poland. He works in the lab of Ewelina Knapska on the neural mechanisms of transmitting emotions between rodents. He graduated from the Faculty of Psychology, University of Warsaw. In his work, he uses such techniques as in vivo optogenetics, immunohistochemistry and electrophysiology.